Nature Communications (Feb 2018)
Genome-wide tracking of dCas9-methyltransferase footprints
- Christina Galonska,
- Jocelyn Charlton,
- Alexandra L. Mattei,
- Julie Donaghey,
- Kendell Clement,
- Hongcang Gu,
- Arman W. Mohammad,
- Elena K. Stamenova,
- Davide Cacchiarelli,
- Sven Klages,
- Bernd Timmermann,
- Tobias Cantz,
- Hans R. Schöler,
- Andreas Gnirke,
- Michael J. Ziller,
- Alexander Meissner
Affiliations
- Christina Galonska
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- Jocelyn Charlton
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- Alexandra L. Mattei
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- Julie Donaghey
- Department of Stem Cell and Regenerative Biology, Harvard University
- Kendell Clement
- Department of Stem Cell and Regenerative Biology, Harvard University
- Hongcang Gu
- Broad Institute of MIT and Harvard
- Arman W. Mohammad
- Broad Institute of MIT and Harvard
- Elena K. Stamenova
- Broad Institute of MIT and Harvard
- Davide Cacchiarelli
- Broad Institute of MIT and Harvard
- Sven Klages
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- Bernd Timmermann
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- Tobias Cantz
- Translational Hepatology and Stem Cell Biology, Hannover Medical School
- Hans R. Schöler
- Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine
- Andreas Gnirke
- Broad Institute of MIT and Harvard
- Michael J. Ziller
- Department for Translational Psychiatry, Max Planck Institute of Psychiatry
- Alexander Meissner
- Department of Genome Regulation, Max Planck Institute for Molecular Genetics
- DOI
- https://doi.org/10.1038/s41467-017-02708-5
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 9
Abstract
Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.
