A TurboID-based proximity labelling approach for identifying the DNA-binding proteins

Abstract

Summary: Biotin proximity labeling is a technique based on the TurboID enzyme that can be used to capture weak or dynamic interactions that had previously not been used to map proteins interacting with a specific DNA sequence. Here, we present a protocol for identifying specific DNA-sequence-binding proteins. We describe steps for biotin labeling of DNA-binding proteins, protein enrichment and sodium dodecyl sulfate polyacrylamide gel electrophoresis separation, and proteomic analysis.For complete details on the use and execution of this protocol, please refer to Wei et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Molecular/Chemical Probes
• Protein Biochemistry
• Proteomics
• Mass Spectrometry