The Effect of Treadmill Exercise on Expression of α-synuclein and miRNA in MPTP Induced-mouse Models of Parkinson’s Disease

Abstract

PURPOSE The goal of this study was to demonstrate whether treadmill exercise have an effect on the level of α-synuclein and microRNA (miRNA) in striatum of Parkinson’s Disease (PD) induced mouse models. METHODS To induced mouse model of PD, administered to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 25 mg/kg) and Probenecid (250 mg/kg) by intraperitoneal injections, 2 times per week during 5 weeks. The groups were randomly divided into 3 groups: CON (C57BL/6 control, n=7), MPTP-C (MPTP control, n=7), and ME (MPTP+Exercise, n=7) and treadmill exercise was performed for 8 weeks (10 m/min, 60 min/day, 5 days/week). RESULTS The result show that treadmill exercise significantly improved locomotor function in ME group compared with MPTP-C group in rotarod performance test. Moreover, treadmill exercise was significantly increased levels of miR-153 as regulating SNCA mRNA in ME group compared with MPTP-C group. Conversely, treadmill exercise was significantly decreased levels of SNCA mRNA and α-synuclein in ME group compared with MPTP-C group. We also found that treadmill exercise was significantly increased levels of tyrosine hydroxylase in ME group compared with MPTP-C group. CONCLUSIONS Taken together, these results indicated that treadmill exercise could ameliorate pathologic features of PD via regulating miRNA against MPTP induced mouse model of PD.

Keywords

• parkinson’s disease
• mptp
• α-synuclein
• tyrosine hydroxylase
• treadmill exercise