Periodontal Ligament Fibroblast Cell Viability Following Treatment with Different Concentrations of Green Tea, Aloe Vera and a Mixture of their Extracts

Abstract

Objectives: Various studies have identified green tea and Aloe vera as a suitable medium for avulsed teeth. The aim of this study was to evaluate and compare the viability of periodontal ligament (PDL) fibroblasts following treatment with the extracts of these two plants and their mixture. Materials and Methods: Human PDL fibroblasts were purchased and treated with different concentrations of Aloe vera, green tea, and a combination of these two extracts. Hank’s balanced salt solution and culture medium were employed as positive and negative controls, respectively. Viability was assessed using the MTT assay. Two-way ANOVA and post-hoc tests were used for statistical analysis (P<0.05). Results: There was a significant difference in PDL fibroblast viability between different concentrations of the extracts. Higher concentrations of green tea and the combination of the two extracts significantly increased cell viability. Higher concentrations of Aloe vera had the least positive effect on maintaining the viability of these cells. Conclusion: If confirmed by further studies, the combination of Aloe vera and green tea extracts might be considered as a suitable media for different purposes like storing avulsed teeth.

Keywords

• Fibroblasts
• Periodontal ligament
• Aloe
• Tea
• Cell survival