High prevalence of blaCTX‐M‐15 type extended‐spectrum beta‐lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction

Abstract

Abstract One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended‐spectrum cephalosporin‐ (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty‐four ESBL‐producing Escherichia coli and five ESBL‐producing Klebsiella pneumoniae isolates were identified in 52 of the samples, of which 52 E. coli and 4 K. pneumoniae yielded passed sequencing results. Fifty of the E. coli had ESBL phenotype and genotype harboring blaCTX‐M genes, of which 88.5% (n = 46) were the blaCTX‐M‐15 gene, commonly found on the African continent. Furthermore, the genetic context around blaCTX‐M‐15 was similar between isolates, being colocalized with ISKpn19. In contrast, cgMLST analysis of the E. coli harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing E. coli populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL E. coli‐prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic‐resistance genes.

Keywords

• antibiotic resistance
• environmental microbiology
• infectious agents
• microbial ecology
• microbiological‐based health strategies