Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation

Saiwei Zhang; Xiaohua Fu; Ya-nan Wang; Lei Wang

STAR Protocols (Jun 2023)

Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation

Saiwei Zhang,
Xiaohua Fu,
Ya-nan Wang,
Lei Wang

Affiliations

Saiwei Zhang: Shanghai Academy of Environmental Sciences, Shanghai 200233, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
Xiaohua Fu: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China
Ya-nan Wang: Qingdao Solid Waste Pollution Control and Resource Engineering Research Center, College of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China
Lei Wang: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Corresponding author

Journal volume & issue: Vol. 4, no. 2
p. 102287

Abstract

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Summary: Here, we present a protocol to analyze the inhibition of self-generated extracellular free organic carbon (EFOC) on CO2 fixation by chemoautotrophic bacteria. We detail the construction and operation of membrane reactor, followed by a simulation experiment to verify the inhibition of EFOC on CO2 fixation. We further describe the analysis of main inhibitory components in EFOC and measurement of abundance and transcription level of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) gene to clarify the mechanism of the main inhibition components on CO2 fixation.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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