In Vitro Antibacterial Potential of <i>Salix babylonica</i> Extract against Bacteria that Affect <i>Oncorhynchus mykiss</i> and <i>Oreochromis</i> spp.
Lenin Rangel-López,
Adrian Zaragoza-Bastida,
Benjamín Valladares-Carranza,
Armando Peláez-Acero,
Carolina G. Sosa-Gutiérrez,
Helal F. Hetta,
Gaber El-Saber Batiha,
Ali Alqahtani,
Nallely Rivero-Perez
Affiliations
Lenin Rangel-López
Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinariay Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, Mexico
Adrian Zaragoza-Bastida
Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinariay Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, Mexico
Benjamín Valladares-Carranza
Facultad de Medicina Veterinaria y Zootecnia Universidad Autónoma del Estado de México, El Cerrillo Piedras Blancas, Toluca 50295, Mexico
Armando Peláez-Acero
Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinariay Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, Mexico
Carolina G. Sosa-Gutiérrez
Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinariay Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, Mexico
Helal F. Hetta
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt
Gaber El-Saber Batiha
Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt
Ali Alqahtani
Department of Pharmacology, College of Pharmacy, King Khalid University, Guraiger, Abha 62529, Saudi Arabia
Nallely Rivero-Perez
Instituto de Ciencias Agropecuarias, Área Académica de Medicina Veterinariay Zootecnia, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, Mexico
Aquaculture development is limited by bacteria associated with several diseases; antibiotics are used for the treatment of these affections, but bacteria have developed resistance to these drugs. It is important to develop effective treatments that allow the production of antibiotic-free food. The aim of the present study is to evaluate the in vitro antibacterial effects of Salix babylonica hydro-alcoholic extract (SbHE) against Aeromonas hydrophila, Listonella anguillarum, Edwarsiella tarda, and Streptococcus iniae, bacteria that affect Oncorhynchus mykiss and Oreochromis spp. production. SbHE was obtained through the maceration technique. Reference strains were used and their sensitivity to antibiotics was determined. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SbHE were determined. Results showed that three of four evaluated bacteria were multidrug resistant, except S. iniae. SbHE showed antibacterial activity against all bacteria. Results indicate an MIC of 1.56 to 25 mg/mL and an MBC of 3.12 to 100 mg/mL. The greatest inhibitory activity occurred against L. anguillarum obtaining a MIC of 1.56 mg/mL and an MBC of 3.12 mg/mL. Results indicate that SbHE has bactericidal activity against A. hydrophila, L.anguilalurm, and S. iniae as well as bacteriostatic activity against E. tarda and could be an alternative treatment against these bacteria.
