Chromosome-Level Genome Reveals the Origin of Neo-Y Chromosome in the Male Barred Knifejaw Oplegnathus fasciatus
Yongshuang Xiao,
Zhizhong Xiao,
Daoyuan Ma,
Chenxi Zhao,
Lin Liu,
Hao Wu,
Wenchao Nie,
Shijun Xiao,
Jing Liu,
Jun Li,
Angel Herrera-Ulloa
Affiliations
Yongshuang Xiao
Center for Ocean Mega-Science, The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Zhizhong Xiao
Center for Ocean Mega-Science, The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Daoyuan Ma
Center for Ocean Mega-Science, The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Chenxi Zhao
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China
Lin Liu
Wuhan Frasergen Bioinformatics Co., Ltd. East Lake High-Tech Zone, Wuhan, China
Hao Wu
Wuhan Frasergen Bioinformatics Co., Ltd. East Lake High-Tech Zone, Wuhan, China
Wenchao Nie
Wuhan Frasergen Bioinformatics Co., Ltd. East Lake High-Tech Zone, Wuhan, China
Shijun Xiao
College of Plant Protection, Jilin Agriculture University, Changchun, Jilin, China; School of Computer Science and Technology, Wuhan University of Technology, Wuhan, China; Corresponding author
Jing Liu
Center for Ocean Mega-Science, The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author
Jun Li
Center for Ocean Mega-Science, The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Corresponding author
Angel Herrera-Ulloa
Escuela de Ciencias Biológicas, Universidad Nacional, San José, Costa Rica
Summary: The barred knifejaw, Oplegnathus fasciatus, is characterized by an X1X2Y system with a neo-Y chromosome for males. Here, a chromosome-level genome was assembled to investigate the origin of neo-Y chromosome to the male O. fasciatus. Twenty-three chromosomes corresponding to the male karyotypes were scaffolded to 762-Mb genome with a contig N50 length of 2.18 Mb. A large neo-Y chromosome (Ch9) in the male O. fasciatus genome was also assembled and exhibited high identity to those of the female chromosomes Ch8 and Ch10. Chromosome rearrangements events were detected in the neo-chromosome Ch9. Our results suggested that a centric fusion of acrocentric chromosomes Ch8 and Ch10 should be responsible for the formation of the X1X2Y system. The high-quality genome will not only provide a solid foundation for further sex-determining mechanism research in the X1X2Y system but also facilitate the artificial breeding aiming to improve the yield and disease resistance for Oplegnathus. : Marine Organism; Biological Sciences; Genetics; Genomics; Evolutionary Biology Subject Areas: Marine Organism, Biological Sciences, Genetics, Genomics, Evolutionary Biology
