Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry

Luigi D’Ascenzo; Anna M. Popova; Scott Abernathy; Kai Sheng; Patrick A. Limbach; James R. Williamson

doi:10.1038/s41467-022-30057-5

Nature Communications (May 2022)

Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry

Luigi D’Ascenzo,
Anna M. Popova,
Scott Abernathy,
Kai Sheng,
Patrick A. Limbach,
James R. Williamson

Affiliations

Luigi D’Ascenzo: Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Anna M. Popova: Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Scott Abernathy: Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati
Kai Sheng: Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Patrick A. Limbach: Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati
James R. Williamson: Department of Integrative Structural and Computational Biology, The Scripps Research Institute

DOI: https://doi.org/10.1038/s41467-022-30057-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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RNA modifications represent a critical aspect of RNA biology that is not well suited to sequencing methods. Here, the authors provide a software tool for automated analysis of RNA tandem mass spectra with full support of modifications, isotope labelling, and control of false discovery rate.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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