Salt stress responses and SNP‐based phylogenetic analysis of Thai rice cultivars

Abstract

Abstract Genetic diversity is important for developing salt‐tolerant rice (Oryza sativa L.) cultivars. Certain Thai rice accessions display salt tolerance at the adult or reproductive stage, but their response to salinity at the seedling stage is unknown. In this study, a total of 10 rice cultivars/line, including eight Thai cultivars and standard salt‐tolerant cultivar and susceptible line, were screened using a hydroponic system to identify salt‐tolerant genotypes at the seedling stage. Different morphophysiological indicators were used to classify tolerant and susceptible genotypes. Phylogenetic analyses were performed to determine the evolutionary relationships between the cultivars. Results showed that ‘Lai Mahk’, ‘Jao Khao’, ‘Luang Pratahn’, and ‘Ma Gawk’ exhibited salt stress tolerance comparable with the standard salt‐tolerance check ‘Pokkali’. Whole‐exome single‐nucleotide polymorphism (SNP)‐based phylogenetic analysis showed that the Thai rice cultivars were monophyletic and distantly related to Pokkali and IR29. Lai Mahk and Luang Pratahn were found closely related when using the whole‐exome SNPs for the analysis. This is also the case for the analysis of SNPs from 164 salt‐tolerance genes and transcription regulatory genes. The tolerant cultivars shared the same haplotype for 16 genes. Overall, the findings of this study identified four salt‐stress‐tolerant Thai rice cultivars, which could be used in rice breeding programs for salinity tolerance.