Synthesis, characterization, anticancer and DNA photocleavage study of novel quinoline Schiff base and its metal complexes

Abstract

This work reports synthesis of novel Quinoline Schiff base by condensation of 2-hydroxyquinoline-3-carbaldehyde and 4-methylbenzenesulfonohydrazide. Prepared Schiff base was further used for the formation of metal complexes with Cu (II), Ni (II), Co (II) & Cd (II). The complete formation of Schiff base and its metal complexes were confirmed by spectroscopic techniques i.e. FT-IR, 1H NMR, 13C NMR, ESI-MS, UV–Visible, and EPR. Low conductivity data indicated that prepared compounds are non-electrolytic. FT-IR data suggested that ligand bears O, N, and O binding sites. Magnetic moment of the compounds indicated that Cu (II), Ni (II), and Co (II) metal complexes are paramagnetic. The synthesized compounds were further evaluated for their In vitro cytotoxicity against the A-549 and MCF-7 cell lines by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide] assay. Among all prepared compounds, Cu-complex has shown prominent results with IC50 values 37.03 and 39.43, for A-549 and MCF-7 cancer cells respectively. Further, most active Cu-complex was subjected for photo-cleavage study with pBR322 DNA.

Keywords

• Quinoline Schiff base
• 1H NMR
• EPR
• Cytotoxicity and DNA photo-cleavage