Cell Cycle Control Gene-Gene Interaction Model in Pregnancy Loss

Abstract

Early stages of individual development are defined by active cell division and differentiation processes. Intercellular signaling molecules as well as proliferation control and DNA stabilizing factors act as basic functional groups. Changes in genome stability maintaining genes functioning can cause the mutation number increase during blastomeric cleavage and differentiation. Thereby the goal of current work was to investigate the frequencies of cell cycle and DNA reparation control genes polymorphic variants in women with normally progressing pregnancies and in women with first trimester pregnancy loss. The present case-control study included 151 women with spontaneous abortion and 134 women with normally progressing pregnancies. Polymorphisms Pro72Arg in TP53, Asp148Glu in APEX1 and Lys751Gln in ERCC2 were detected by allele-specific PCR. When analyzed separately, the allele and genotype distributions of the polymorphisms in the two groups were similar. However, in MDR analysis the interaction between the genotypes TP53, APEX1, XPD showed to be associated to early pregnancy loss.

Keywords

• TP53
• APEX1
• Pregnancy Loss
• Gene Polymorphism
• Gene-Gene Interaction