Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Junheng Lv; Minghua Deng; Zuosen Li; Haishan Zhu; Ziran Wang; Yanling Yue; Zhengan Yang; Junqiang Xu; Shurui Jiang; Wei Zhao; Jing Li; Kai Zhao

Horticultural Plant Journal (Oct 2023)

Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Junheng Lv,
Minghua Deng,
Zuosen Li,
Haishan Zhu,
Ziran Wang,
Yanling Yue,
Zhengan Yang,
Junqiang Xu,
Shurui Jiang,
Wei Zhao,
Jing Li,
Kai Zhao

Affiliations

Junheng Lv: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Minghua Deng: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Zuosen Li: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Haishan Zhu: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Ziran Wang: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Yanling Yue: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Zhengan Yang: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Junqiang Xu: Dian-Tai Engineering Research Center for Characteristic Agriculture Industrialization of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Shurui Jiang: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Wei Zhao: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Jing Li: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Kai Zhao: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Corresponding author. Tel.: +86 010 82109512

Journal volume & issue: Vol. 9, no. 5
pp. 958 – 970

Abstract

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Tomato spotted wilt virus (TSWV) is an important virus that has rapidly spread throughout the world. TSWV seriously hinders the production of tomato (Solanum lycopersicum) and other plants. In order to discover more new genes and metabolites related to TSWV resistance in tomato plants, the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied herein. The tomato TSWV-resistance line YNAU335 (335) and TSWV-susceptible lines NO5 and 96172I (961) were used as the transcriptome and metabolome research materials. Transcriptomic and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation. A total of 3 566, 2 951, and 2 674 differentially expressed genes (DEGs) were identified in lines 335, NO5, and 961, respectively. Meanwhile, 208, 228, and 273 differentially accumulated metabolites (DAMs) were identified in lines 335, NO5, and 961, respectively. In line 335, the number of DEGs was the highest, but the number of DAMs was lowest. Furthermore, 903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred lines. The 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis pathways. In addition, many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV response. These results provide new insights into TSWV resistance mechanisms.

Published in Horticultural Plant Journal

ISSN: 2095-9885 (Print); 2468-0141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Plant culture
Website: https://www.keaipublishing.com/en/journals/horticultural-plant-journal/

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