Nature Communications (Jan 2021)
Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8
- Qin Gong,
- Kim Robinson,
- Chenrui Xu,
- Phuong Thao Huynh,
- Kelvin Han Chung Chong,
- Eddie Yong Jun Tan,
- Jiawen Zhang,
- Zhao Zhi Boo,
- Daniel Eng Thiam Teo,
- Kenneth Lay,
- Yaming Zhang,
- John Soon Yew Lim,
- Wah Ing Goh,
- Graham Wright,
- Franklin L. Zhong,
- Bruno Reversade,
- Bin Wu
Affiliations
- Qin Gong
- School of Biological Sciences, Nanyang Technological University
- Kim Robinson
- Skin Research Institute (SRIS), Agency of Science Technology and Research (A*STAR)
- Chenrui Xu
- School of Biological Sciences, Nanyang Technological University
- Phuong Thao Huynh
- School of Biological Sciences, Nanyang Technological University
- Kelvin Han Chung Chong
- School of Biological Sciences, Nanyang Technological University
- Eddie Yong Jun Tan
- School of Biological Sciences, Nanyang Technological University
- Jiawen Zhang
- School of Biological Sciences, Nanyang Technological University
- Zhao Zhi Boo
- School of Biological Sciences, Nanyang Technological University
- Daniel Eng Thiam Teo
- Institute of Molecular and Cell Biology, Agency of Science Technology and Research (A*STAR)
- Kenneth Lay
- Institute of Molecular and Cell Biology, Agency of Science Technology and Research (A*STAR)
- Yaming Zhang
- School of Biological Sciences, Nanyang Technological University
- John Soon Yew Lim
- Skin Research Institute (SRIS), Agency of Science Technology and Research (A*STAR)
- Wah Ing Goh
- Skin Research Institute (SRIS), Agency of Science Technology and Research (A*STAR)
- Graham Wright
- Institute of Molecular and Cell Biology, Agency of Science Technology and Research (A*STAR)
- Franklin L. Zhong
- Skin Research Institute (SRIS), Agency of Science Technology and Research (A*STAR)
- Bruno Reversade
- Institute of Molecular and Cell Biology, Agency of Science Technology and Research (A*STAR)
- Bin Wu
- School of Biological Sciences, Nanyang Technological University
- DOI
- https://doi.org/10.1038/s41467-020-20319-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
NLRP1 and CARD8 are two recently described sensor proteins for the human inflammasome complex. Here, the authors present the cryo-EM CARD filament structures of the NLRP1 and CARD8 activating domains, which reveal how NLRP1 and CARD8 discriminate between ASC and pro-caspase-1. They further propose a two-step model for NLRP1 activation.
