Nanomaterials (Apr 2013)

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

  • Po-Jung Jimmy Huang,
  • Juewen Liu

DOI
https://doi.org/10.3390/nano3020221
Journal volume & issue
Vol. 3, no. 2
pp. 221 – 228

Abstract

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Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.

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