Identification of proteins and miRNAs that specifically bind an mRNA in vivo

Kathrin Theil; Koshi Imami; Nikolaus Rajewsky

doi:10.1038/s41467-019-12050-7

Nature Communications (Sep 2019)

Identification of proteins and miRNAs that specifically bind an mRNA in vivo

Kathrin Theil,
Koshi Imami,
Nikolaus Rajewsky

Affiliations

Kathrin Theil: Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Koshi Imami: Proteome Dynamics, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Nikolaus Rajewsky: Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

DOI: https://doi.org/10.1038/s41467-019-12050-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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The entire mRNA interactome capture has been established but identification of specific mRNA-binding proteins has been challenging. Here, the authors developed an in vivo RNA–protein crosslinking and RNA pulldown approach and characterized novel interactors of the C. elegans gld-1 mRNA, including DAZ-1 and miR-84.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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