Nature Communications (Apr 2020)
Multiplex secretome engineering enhances recombinant protein production and purity
- Stefan Kol,
- Daniel Ley,
- Tune Wulff,
- Marianne Decker,
- Johnny Arnsdorf,
- Sanne Schoffelen,
- Anders Holmgaard Hansen,
- Tanja Lyholm Jensen,
- Jahir M. Gutierrez,
- Austin W. T. Chiang,
- Helen O. Masson,
- Bernhard O. Palsson,
- Bjørn G. Voldborg,
- Lasse Ebdrup Pedersen,
- Helene Faustrup Kildegaard,
- Gyun Min Lee,
- Nathan E. Lewis
Affiliations
- Stefan Kol
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Daniel Ley
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Tune Wulff
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Marianne Decker
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Johnny Arnsdorf
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Sanne Schoffelen
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Anders Holmgaard Hansen
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Tanja Lyholm Jensen
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Jahir M. Gutierrez
- The Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of Medicine
- Austin W. T. Chiang
- The Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of Medicine
- Helen O. Masson
- The Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of Medicine
- Bernhard O. Palsson
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Bjørn G. Voldborg
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Lasse Ebdrup Pedersen
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Helene Faustrup Kildegaard
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Gyun Min Lee
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Building 220, Kemitorvet
- Nathan E. Lewis
- The Novo Nordisk Foundation Center for Biosustainability at the University of California, San Diego, School of Medicine
- DOI
- https://doi.org/10.1038/s41467-020-15866-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Host cell proteins can contaminate biotherapeutics and compromise and degrade their quality. Here the authors use modelling and CRISPR to delete secreted host proteins in CHO cells, leading to improved monoclonal antibody production and purity.
