UHPLC-HRMS/MS Chemical Fingerprinting of the Bioactive Partition from Cultivated <i>Piper aduncum</i> L.
Adélia Viviane de Luna,
Thayssa da Silva Ferreira Fagundes,
Ygor Jessé Ramos,
Marlon Heggdorne de Araújo,
Michelle Frazão Muzitano,
Sanderson Dias Calixto,
Thatiana Lopes Biá Ventura Simão,
George Azevedo de Queiroz,
Elsie Franklin Guimarães,
André Mesquita Marques,
Davyson de Lima Moreira
Affiliations
Adélia Viviane de Luna
Postgraduate Program in Translational Research in Drugs and Medicines, Pharmaceutical Technology Institute, Far-Manguinhos, Fiocruz, Rua Sizenando Nabuco, 100, Manguinhos, Rio de Janeiro 21041-250, RJ, Brazil
Thayssa da Silva Ferreira Fagundes
Botanical Garden Research Institute of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, RJ, Brazil
Ygor Jessé Ramos
Farmácia da Terra Laboratory, Faculty of Pharmacy, Federal University of Bahia, Rua Barão de Jeremoabo, 147, Ondina, Salvador 40170-115, BA, Brazil
Marlon Heggdorne de Araújo
Laboratory of Bioatives Products, Institute of Pharmaceutical Sciences, Federal University of Rio de Janeiro, Rua Alcides da Conceição, 159, Macaé 27933-378, RJ, Brazil
Michelle Frazão Muzitano
Laboratory of Bioatives Products, Institute of Pharmaceutical Sciences, Federal University of Rio de Janeiro, Rua Alcides da Conceição, 159, Macaé 27933-378, RJ, Brazil
Sanderson Dias Calixto
Recenor Biology Laboratory, Center of Biosciences and Biotechnology, State University of North Fluminense Darcy Ribeiro, Rua Alberto Lamego, 2000, Campos dos Goytacazes 28013-602, RJ, Brazil
Thatiana Lopes Biá Ventura Simão
Recenor Biology Laboratory, Center of Biosciences and Biotechnology, State University of North Fluminense Darcy Ribeiro, Rua Alberto Lamego, 2000, Campos dos Goytacazes 28013-602, RJ, Brazil
George Azevedo de Queiroz
Botanical Garden Research Institute of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, RJ, Brazil
Elsie Franklin Guimarães
Botanical Garden Research Institute of Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, Rio de Janeiro 22460-030, RJ, Brazil
André Mesquita Marques
Postgraduate Program in Translational Research in Drugs and Medicines, Pharmaceutical Technology Institute, Far-Manguinhos, Fiocruz, Rua Sizenando Nabuco, 100, Manguinhos, Rio de Janeiro 21041-250, RJ, Brazil
Davyson de Lima Moreira
Postgraduate Program in Translational Research in Drugs and Medicines, Pharmaceutical Technology Institute, Far-Manguinhos, Fiocruz, Rua Sizenando Nabuco, 100, Manguinhos, Rio de Janeiro 21041-250, RJ, Brazil
Piper aduncum L. is widely distributed in tropical regions and the ethnobotanical uses of this species encompass medicinal applications for the treatment of respiratory, antimicrobial, and gynecological diseases. Chemical studies reveal a diverse array of secondary metabolites, including terpenes, flavonoids, and prenylated compounds. Extracts from P. aduncum have shown antibacterial, antifungal, and larvicidal activities. Our study explores the activity of extracts and partitions against Mycobacterium tuberculosis H37Rv, as well as the chemical diversity of the bioactive partition. This marks the first investigation of the bioactive partition of P. aduncum from agroecological cultivation. The ethyl acetate partition from the ethanolic leaf extract (PAEPL) was found to be the most active. PAEPL was subjected to column chromatography using Sephadex LH-20 and the obtained fractions were analyzed using UHPLC-HRMS/MS. The MS/MS data from the fractions were submitted to the online GNPS platform for the generation of the molecular network, which displayed 1714 nodes and 167 clusters. Compounds were identified via manual inspection and different libraries, allowing the annotation of 83 compounds, including flavonoids, benzoic acid derivatives, glycosides, free fatty acids, and glycerol-esterified fatty acids. This study provides the first chemical fingerprint of an antimycobacterial sample from P. aduncum cultivated in an agroecological system.
