Antibacterial enhancement of antibiotic activity by Enterolobium contortisiliquum (Vell.) Morong

Abstract

Objective: To identify the main chemical classes of compounds from aqueous extract of Enterolobium contortisiliquum (E. contortisiliquum) seed bark and to evaluate its antibacterial activity, as well as its potential to increase the activity of antibiotics against strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Methods: Different classes of compounds in the aqueous extract of E. contortisiliquum were evaluated based on the visual changes in the coloration and the formation of precipitate after the addition of specific reagents. The antibacterial activity of the extract and its potential to increase of antibiotic activity of antibiotics drugs, gentamicin and norfloxacin was determined by using the microdilution method. Results: Our results demonstrated that the following secondary metabolites were presented in E. contortisiliquum seed bark: flavones, flavonols, xanthones, flavononols, chalcones, aurones, flavones and catechins. The extract itself had very low antibacterial activity against all bacterial strains tested (MIC ≥ 1 024 μg/mL), but there was an increase in the antibiotic activity of gentamicin and norfloxacin when combined in the sub-inhibitory concentration (i.e., MIC/8). Conclusions: Our data suggests that E. contortisiliquum seed bark may be an alternative source for new drugs with the potential to increase antibiotic activity against different strains of bacteria.

Keywords

• Antibiotic activity
• Enhancement
• Synergism
• Chemical compounds
• Bioactivity