The possible effects of sodium fluoroscein to primary cell culture sampling in glioblastoma surgery

Abstract

Introduction: Primary glioblastoma (GBM) cell cultures have become a very important tool for future research due to the patient-specific genetic makeup of GBM cells and different treatment response variations. In sodium fluorescein-guided surgeries, regions sampled for primary cell culture are also stained with FL. However, the effects of FL on GBM cell cultures are unknown. In our study, the effect of FL on GBM cell cultures after a possible contamination was investigated. Resarch Question: Does sodium fluorescein have an effect on glioblastoma cell cultures? If there is, should sodium fluorescein be used after primary cell culture sampling when performing sodium fluorescein-guided glioblastoma surgery? Methods: Standard cell culture techniques were used to generate GBM cell lines, which were then split into control and FL treated groups. Cell counts, cell viability, cellular aging, and beta-galactosidase analyses were compared between these groups. Cells' microscopic pictures were also assessed. Results: Our study reveals that FL-applied cultures had fewer cells overall and more s beta-galactosidase activity than control groups. The notion that FL may have an impact on the environment of GBM cell cultures is thus raised by our main findings regarding the use of FL and its effects on cell cultures. Conclusion: To prevent adverse effects on the growth of primary GBM cell cultures, neurosurgeons might think about using FL after sampling the tumor tissue during operations.

Keywords

• Sodium fluorescein
• Glioblastoma
• Cell culture