Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus

Sara Miellet; Mitchell St Clair-Glover; Marnie Maddock; Mirella Dottori

Stem Cell Research (Mar 2024)

Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus

Sara Miellet,
Mitchell St Clair-Glover,
Marnie Maddock,
Mirella Dottori

Affiliations

Sara Miellet: Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, Australia
Mitchell St Clair-Glover: Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, Australia
Marnie Maddock: Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, Australia
Mirella Dottori: Corresponding author.; Molecular Horizons, School of Medical, Indigenous and Health Sciences University of Wollongong, Australia

Journal volume & issue: Vol. 75
p. 103312

Abstract

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The pro-neural transcription factor neurogenin-2 (NGN2) possesses the ability to rapidly and effectively transform stem cells into fully operational neurons. Here we report the successful generation of a modified H9 human embryonic H9 stem cell line containing a doxycycline (DOX) inducible NGN2 expression construct featuring a floxed Blasticidin/mApple selection module in the safe-harbor locus CLYBL. This cell line retains its pluripotent state in the absence of DOX, yet readily transitions into a neuronal state upon DOX introduction.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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