Generation of an integration-free induced pluripotent stem cell line (UNC001-A) from blood of a healthy individual

Sarahi G. Molina; Alvaro A. Beltran; Adriana S. Beltran

Stem Cell Research (Dec 2020)

Generation of an integration-free induced pluripotent stem cell line (UNC001-A) from blood of a healthy individual

Sarahi G. Molina,
Alvaro A. Beltran,
Adriana S. Beltran

Affiliations

Sarahi G. Molina: Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
Alvaro A. Beltran: Department of Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
Adriana S. Beltran: Human Pluripotent Stem Cell Core, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Department of Pharmacology at the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Corresponding author at: Department of Pharmacology at the University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.

Journal volume & issue: Vol. 49
p. 102015

Abstract

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Induced pluripotent stem cells (iPSCs) generated from young, healthy individuals are valuable tools for investigating molecular disease mechanisms during the early development of the brain vasculature. We generated an iPSC line from peripheral blood mononuclear cells (PBMCs) isolated from a healthy 13-yeard old female donor using the Sendai virus. The iPSCs differentiated into endothelial cells, astrocytes, and neurons. This iPSC line can serve as a healthy reference control for comparative studies in drug development and modeling the early onset of Cerebral Cavernous Malformation (CCM).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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