Effects of UBE2N Knockdown on Radiosensitivity of Human Laryngeal Squamous Cell Carcinoma hep-2 Cells

Abstract

Objective To investigate the role of UBE2N in the radiosensitivity of laryngocarcinoma hep-2 cells. Methods qPCR and Western blot were used to investigate the silencing efficiency of UBE2N siRNA in hep-2 cells. RNA interference was used to inhibit the expression of UBE2N. After being irradiated 0, 2, 4, 6, and 8 Gy X-rays irradiation, cell proliferation was detected by CCK8 assay, cell cycle and apoptosis were detected by flow cytometry, and the ability of propagation was detected by clonal formation assay. Results UBE2N siRNA significantly inhibited the expression of UBE2N at the mRNA and protein levels in hep-2 cells. UBE2N silence significantly enhanced the proliferation of hep-2 cells. After UBE2N silencing and certain doses of radiation, the proliferation ability of hep-2 cells was enhanced, the S stage proportion was increased while, the G2 stage proportion was decreased, the apoptosis rate was decreased while the clonal formation ability was enhanced, compared with the control group (all P < 0.05). Conclusion As a tumor suppressor gene, UBE2N enhances the radiosensitivity of laryngeal squamous cell carcinoma by regulating cell proliferation, cell cycle, apoptosis and clonal formation ability.

Keywords

• ube2n
• laryngeal squamous cell carcinoma
• radiosensitivity