Bacterially mediated release and mobilization of As/Fe coupled to nitrate reduction in a sediment environment

Abstract

Metal-reducing bacteria play an important role in the release and mobilization of arsenic from sediments into groundwater. This study aimed to investigate the influence of nitrate on arsenic bio-release. Microcosm experiments consisting of high arsenic sediments and indigenous bacterium Bacillus sp. D2201 were conducted and the effects of nitrate on the mobilization of As/Fe determined. The results show arsenic release is triggered by iron reduction, which is regulated by nitrate. Increasing the nitrate concentration from 0 to 1 and 3 mM decreased Fe(III) reduction by 62.5% and 16.9% and decreased As(V) bio-release by 41.5% and 85.5%, respectively. Moreover, the results of step-wise Wenzel sequential extractions indicate nitrate addition prevents the transformation of poorly crystalline iron oxides to well crystalline iron oxides. Overall, nitrate appears to have a dual effect, inhibiting both iron reduction and arsenic release by incubation strain D2201. This study offers new insights regarding the biogeochemistry of arsenic in groundwater systems.

Keywords

• Metal-reducing bacteria
• Nitrate
• Bio-release of As/Fe
• High arsenic sediments