Protoplast Isolation in Lupin Lupinus spp

Abstract

The aim of this wprk was to find out a method for isolation of+ protoplasts from various lupin Lupinus spp species. Al 10 ml I , CaCl2. 2H20 0.103 g I , Cellulase Onozuka R-10 1.5 %, Macerozyme R-10 0.1%, mannitol 8.96 % and BSA 0.1% have been found to be a suitable enzyme mixt yre for isolating protoplasts from the leaves of glasshouse-grown lupin species. Digestion in the dark in the refrigerator C for 14-15 hours followed by incubation at room temperature for 30- 60 minutes and then shaking at 70 rpm at 25 C for 30 minutes released a high quality and number of protoplasts in all lupin species examined.

Keywords

• lupin
• lupinus
• protoplast
• izolasyon
• lupinus
• lupin
• protoplast
• isolation