Antioxidant and Antiproliferative Activity of the Ethanolic Extract of <i>Equisetum myriochaetum</i> and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin
Fabián Olazarán-Santibañez,
Gildardo Rivera,
Venancio Vanoye-Eligio,
Arturo Mora-Olivo,
Gabriel Aguirre-Guzmán,
Mónica Ramírez-Cabrera,
Eder Arredondo-Espinoza
Affiliations
Fabián Olazarán-Santibañez
Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, Mexico
Gildardo Rivera
Centro de Biotecnología Genómica, Laboratorio de Biotecnología Farmacéutica, Instituto Politécnico Nacional, Reynosa 88710, Mexico
Venancio Vanoye-Eligio
Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, Mexico
Arturo Mora-Olivo
Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87019, Mexico
Gabriel Aguirre-Guzmán
Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87000, Mexico
Mónica Ramírez-Cabrera
Laboratorio de Farmacología Molecular y Modelos Biológicos, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo Leon, Av Guerrero s/n, Treviño, C.P., Monterrey 64570, Mexico
Eder Arredondo-Espinoza
Laboratorio de Farmacología Molecular y Modelos Biológicos, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo Leon, Av Guerrero s/n, Treviño, C.P., Monterrey 64570, Mexico
Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 μg/mL against SiHa cells.
