A Comparison between Different Acquisition Modes for FT-IR Spectra Collection from Human Cell Lipid Extracts

Abstract

Lipids are organic compounds that contribute to numerous cellular functions. Fourier Transform Infrared spectroscopy can be particularly useful in investigating the biochemical features of the lipid content of cells and their changes induced by interaction with physicochemical external agents. In the present work, we aim to investigate the extract of lipids from human cells to compare the results obtained by using two different geometries: transmission and attenuated total reflectance. Multiple acquisitions of spectra were carried out and statistical criteria were applied for monitoring and comparing them. The positive and negative aspects of the two examined acquisition modes are presented and discussed.

Keywords

• FT-IR spectroscopy
• transmission
• ATR
• lipids
• hepatocarcinoma cells