A cardiovascular phenotype in warfarin-resistant Vkorc1 mutant rats☆

Abstract

Background: The inhibition of the vitamin K cycle by warfarin promotes arterial calcification in the rat. Conceivably, genetically determined vitamin K deficiency owing to a mutant epoxide reductase subcomponent 1 (Vkorc1) gene, a key component of the vitamin K cycle, might also promote arterial calcification. In the absence of an available Vkorc1 gene knockout model we used a wild-derived Vkorc1 mutant rat strain (Rattus norvegicus) to explore the validity of this hypothesis. Methods: We provide histopathological descriptions of a naturally occurring Vkorc1 gene knockdown: wild-derived lab-reared rats that are resistant to the anticoagulant warfarin owing to a non-synonymous mutation in the Vkorc1 gene (Vkorc1Y→C), which, in vitro, reduces the basal activity of the vitamin K epoxide reductase enzyme complex by ~52%. H&E stained sections of heart and kidney were compared between homozygous Vkorc1Y→C/Y→C, heterozygous Vkorc1Y→C/+ and wildtype Vkorc1+/+ rats of both sexes. Results: We observed that the aorta of the heart was mineralized in the Vkorc1Y→C/Y→C male rats but lesions were virtually absent from Vkorc1Y→C/+ and Vkorc1+/+ male and all female rats. The renal arteries were mineralized in Vkorc1Y→C/Y→C and Vkorc1Y→C/+ mutant rats, regardless of sex. Conclusions: Results support a hypothesis that posits that Vkorc1 genetic polymorphisms reducing basal enzyme activity could affect cardiovascular health, with dependencies on genotype, sex, and tissue. The undercarboxylation of the vitamin K-dependent Matrix Gla protein may be the crucial component of the pathway promoting this mineralization.

Keywords

• Aorta
• Mineralization
• Renal artery
• Urolith
• Vitamin K epoxide reductase
• Warfarin