Acta Cirúrgica Brasileira (May 2021)

Three-dimensional printed poly (L-lactide) and hydroxyapatite composite for reconstruction of critical bone defect in rabbits

  • Bruno Watanabe Minto,
  • Arícia Gomes Sprada,
  • José Aloizio Gonçalves Neto,
  • Brenda Mendonça de Alcântara,
  • Thiago André Salvitti de Sá Rocha,
  • Ana Carolina Valentim Hespanha,
  • Carolina Quarterone,
  • Maressa da Rocha Sartori,
  • Alessandre Hataka,
  • Ricardo Andres Ramirez Uscategui,
  • Luis Gustavo Gosuen Gonçalves Dias

DOI
https://doi.org/10.1590/acb360404
Journal volume & issue
Vol. 36, no. 4

Abstract

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ABSTRACT Purpose To use a 3D printed poly (L-lactide) acid (PLLA) and hydroxyapatite (HA) composite as a bone substitute for reconstruction of a critical bone defect in the radius of rabbits. Methods A 1.5 cm ostectomy was performed in the radial diaphysis of 60 New Zealand white rabbits. The rabbits were divided into three groups according to surgical treatment of the bone defect (group I – control, group II – bone graft, group III – 3D PLLA). Each group was divided into four subgroups with different radiographic and histopathologic evaluation times (T1 – 15 days, T2 – 30 days, T3 – 60 days, T4 – 90 days). Results The implant group had greater clinically lameness (p = 0.02), edema (p = 0.007), pain (p = 0.04) and more complications at the surgical site (p = 0.03). Histologically, this group showed greater congestion (p = 0.04), hemorrhage (p = 0.04) and inflammation. Osteogenesis was microscopically similar between days (p = 0.54) and treatments (p = 0.17), even though radiographically, more effective bone healing occurred in the graft group (II), with more callus and bone bridge formation. Conclusions The customization of a 3D PLLA/HA scaffold was successful. However, in animals receiving the polymer-ceramic composite less bone callus and bone bridge was formed compared to the graft group.

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