Genomics Data (Mar 2016)

Genome Sequencing and Annotation of Mycobacterium tuberculosis PR08 strain

  • Mohammad Maaruf Jaafar,
  • Mohd Zakihalani A. Halim,
  • Mohamad Izwan Ismail,
  • Lee Lian Shien,
  • Teh Lay Kek,
  • Ngeow Yun Fong,
  • Norazmi Mohd Nor,
  • Zainul Fadziruddin Zainuddin,
  • Tang Thean Hock,
  • Mohd Nazalan Mohd Najimudin,
  • Mohd Zaki Salleh

Journal volume & issue
Vol. 7
pp. 119 – 120

Abstract

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Mycobacterium tuberculosis is an acid fast bacterial species in the family Mycobacteriaceae and is the causative agent of most cases of tuberculosis. Here, we report the genomic features of Mycobacterium tuberculosis isolated from the cerebrospinal fluid (CSF) of a patient diagnosed with both pulmonary and extrapulmonary tuberculosis (TB). The isolated strain was identified as Mycobacterium tuberculosis PR08 (MTB PR08). Genomic DNA of the MTB PR08 strain was extracted and subjected to whole genome sequencing using MiSeq (Illumina, CA,USA). The draft genome size of MTB PR08 strain is 4,292,364 bp with a G + C content of 65.2%. This strain was annotated to have 4723 genes and 48 RNAs. This whole genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number CP010895. Keywords: Mycobacterium tuberculosis, Genome, Extrapulmonary, CSF