Direct Application of 3-Maleimido-PROXYL for Proving Hypoalbuminemia in Cases of SARS-CoV-2 Infection: The Potential Diagnostic Method of Determining Albumin Instability and Oxidized Protein Level in Severe COVID-19

Ekaterina Georgieva; Vasil Atanasov; Rositsa Kostandieva; Vanya Tsoneva; Mitko Mitev; Georgi Arabadzhiev; Yovcho Yovchev; Yanka Karamalakova; Galina Nikolova

doi:10.3390/ijms24065807

International Journal of Molecular Sciences (Mar 2023)

Direct Application of 3-Maleimido-PROXYL for Proving Hypoalbuminemia in Cases of SARS-CoV-2 Infection: The Potential Diagnostic Method of Determining Albumin Instability and Oxidized Protein Level in Severe COVID-19

Ekaterina Georgieva,
Vasil Atanasov,
Rositsa Kostandieva,
Vanya Tsoneva,
Mitko Mitev,
Georgi Arabadzhiev,
Yovcho Yovchev,
Yanka Karamalakova,
Galina Nikolova

Affiliations

Ekaterina Georgieva: Department of “General and Clinical Pathology, Forensic Medicine, Deontology and Dermatovenerology”, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria
Vasil Atanasov: Forensic Toxicology Laboratory, Military Medical Academy, 3 G. Sofiiski, 1606 Sofia, Bulgaria
Rositsa Kostandieva: Forensic Toxicology Laboratory, Military Medical Academy, 3 G. Sofiiski, 1606 Sofia, Bulgaria
Vanya Tsoneva: Department of Propaedeutics of Internal Medicine and Clinical Laboratory, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria
Mitko Mitev: Department of “Diagnostic Imaging”, University Hospital “Prof. Dr. St. Kirkovich”, 6000 Stara Zagora, Bulgaria
Georgi Arabadzhiev: Department of “Surgery and anesthesiology”, University Hospital “Prof. Dr. St. Kirkovich”, 6000 Stara Zagora, Bulgaria
Yovcho Yovchev: Department of “Surgery and anesthesiology”, University Hospital “Prof. Dr. St. Kirkovich”, 6000 Stara Zagora, Bulgaria
Yanka Karamalakova: Department of “Medical Chemistry and Biochemistry”, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria
Galina Nikolova: Department of “Medical Chemistry and Biochemistry”, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria

DOI: https://doi.org/10.3390/ijms24065807
Journal volume & issue: Vol. 24, no. 6
p. 5807

Abstract

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Oxidative stress and the albumin oxidized form can lead to hypoalbuminemia, which is a predisposing factor for reduced treatment effectiveness and an increased mortality rate in severe COVID-19 patients. The aim of the study is to evaluate the application of free radical 3-Maleimido-PROXYL and SDSL-EPR spectroscopy in the in vitro determination of ox/red HSA in serum samples from patients with SARS-CoV-2 infection. Venous blood was collected from patients intubated (pO2 max values and ΔH0 spectral parameters comparable to those of 3-Maleimido-PROXYL/DMSO. Based on the obtained results, we suggest that the stable nitroxide radical 3-Maleimido-PROXYL can be successfully used as a marker to study oxidized albumin levels in COVID-19.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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