Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Christopher R. Horne; Hariprasad Venugopal; Santosh Panjikar; David M. Wood; Amy Henrickson; Emre Brookes; Rachel A. North; James M. Murphy; Rosmarie Friemann; Michael D. W. Griffin; Georg Ramm; Borries Demeler; Renwick C. J. Dobson

doi:10.1038/s41467-021-22253-6

Nature Communications (Mar 2021)

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Christopher R. Horne,
Hariprasad Venugopal,
Santosh Panjikar,
David M. Wood,
Amy Henrickson,
Emre Brookes,
Rachel A. North,
James M. Murphy,
Rosmarie Friemann,
Michael D. W. Griffin,
Georg Ramm,
Borries Demeler,
Renwick C. J. Dobson

Affiliations

Christopher R. Horne: Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury
Hariprasad Venugopal: Clive and Vera Ramaciotti Centre for Cryo-Electron Microscopy, Monash University
Santosh Panjikar: Australian Synchrotron, ANSTO
David M. Wood: Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury
Amy Henrickson: Department of Chemistry and Biochemistry, University of Lethbridge
Emre Brookes: Department of Chemistry, University of Montana
Rachel A. North: Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury
James M. Murphy: Walter and Eliza Hall Institute of Medical Research
Rosmarie Friemann: Department of Clinical Microbiology, Sahlgrenska University Hospital
Michael D. W. Griffin: Bio21 Molecular Science and Biotechnology Institute, Department of Biochemistry and Pharmacology, University of Melbourne
Georg Ramm: Clive and Vera Ramaciotti Centre for Cryo-Electron Microscopy, Monash University
Borries Demeler: Department of Chemistry and Biochemistry, University of Lethbridge
Renwick C. J. Dobson: Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury

DOI: https://doi.org/10.1038/s41467-021-22253-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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The GntR superfamily is one of the largest families of transcription factors in prokaryotes. Here the authors combine biophysical analysis and structural biology to dissect the mechanism by which NanR — a GntR-family regulator — binds to its promoter to repress the transcription of genes necessary for sialic acid metabolism.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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