Viruses (Mar 2022)

Identification and Characterization of vB_PreP_EPr2, a Lytic Bacteriophage of Pan-Drug Resistant <i>Providencia rettgeri</i>

  • Jaime L. Mencke,
  • Yunxiu He,
  • Andrey A. Filippov,
  • Mikeljon P. Nikolich,
  • Ashton T. Belew,
  • Derrick E. Fouts,
  • Patrick T. McGann,
  • Brett E. Swierczewski,
  • Derese Getnet,
  • Damon W. Ellison,
  • Katie R. Margulieux

DOI
https://doi.org/10.3390/v14040708
Journal volume & issue
Vol. 14, no. 4
p. 708

Abstract

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Providencia rettgeri is an emerging opportunistic Gram-negative pathogen with reports of increasing antibiotic resistance. Pan-drug resistant (PDR) P. rettgeri infections are a growing concern, demonstrating a need for the development of alternative treatment options which is fueling a renewed interest in bacteriophage (phage) therapy. Here, we identify and characterize phage vB_PreP_EPr2 (EPr2) with lytic activity against PDR P. rettgeri MRSN 845308, a clinical isolate that carries multiple antibiotic resistance genes. EPr2 was isolated from an environmental water sample and belongs to the family Autographiviridae, subfamily Studiervirinae and genus Kayfunavirus, with a genome size of 41,261 base pairs. Additional phenotypic characterization showed an optimal MOI of 1 and a burst size of 12.3 ± 3.4 PFU per bacterium. EPr2 was determined to have a narrow host range against a panel of clinical P. rettgeri strains. Despite this fact, EPr2 is a promising lytic phage with potential for use as an alternative therapeutic for treatment of PDR P. rettgeri infections.

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