Evaluation of the diagnosis of helicobacter pylori from stomach biopsy samples by staining methods

Abstract

In this study, it was aimed to retrospectively investigate the presence of H. pylori with Modified Giemsa and Hematoxylin & Eosin in gastric antrum biopsy samples of patients who applied to Ordu University hospital with dyspeptic complaints. Also, cross-sections were stained with Giemsa, Wright's eosin methylene blue and modified Giemsa dyes to compare their effectiveness in diagnosis. The population of the study consisted of 2679 gastric biopsy samples sent to the pathology laboratory between 2014 and 2018. Gastric biopsy samples were screened, and samples stained with Hematoxylin & Eosin, Giemsa and Periodic Acid Schift (PAS) were re-examined for H. pylori In the microscopic examination, the samples were also evaluated in terms of intestinal metaplasia, activation and atrophy. In the study, 37 negative, 31 mildly positive, 31 moderately positive and 31 severely positive samples were randomly selected, in addition to routine staining methods. Selected samples were re-sectioned, stained with Giemsa and Wright's eosin dye, and Mayg Grunwald-Giemsa (MGG) dye, Giemsa and Wright's eosin methods were compared. A total of 2679 patients, 49.15% male and 50.85% female, were included in the study. The mean age of patients aged 17-93 was 50.42±15.32. H. pylori positivity was found to be 46.8% in the study. It was determined in the study that there was a significant association between H. pylori severity and inflammation (p [Med-Science 2023; 12(1.000): 115-21]

Keywords

• h. pylori
• stomach
• antrum biopsy
• ordu