In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors

Shey-Li Lim; Chia Pao Voon; Xiaoqian Guan; Yi Yang; Per Gardeström; Boon Leong Lim

doi:10.1038/s41467-020-17056-0

Nature Communications (Jun 2020)

In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors

Shey-Li Lim,
Chia Pao Voon,
Xiaoqian Guan,
Yi Yang,
Per Gardeström,
Boon Leong Lim

Affiliations

Shey-Li Lim: School of Biological Sciences, University of Hong Kong
Chia Pao Voon: School of Biological Sciences, University of Hong Kong
Xiaoqian Guan: School of Biological Sciences, University of Hong Kong
Yi Yang: Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology
Per Gardeström: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University
Boon Leong Lim: School of Biological Sciences, University of Hong Kong

DOI: https://doi.org/10.1038/s41467-020-17056-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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NADP(H) and NAD(H) are crucial energy molecules in plant metabolism. Here, via the use of circularly permutated fluorescent protein sensors, the authors demonstrate dynamic changes in NADPH and the NADH/NAD+ ratio during photosynthesis and photorespiration at the subcellular level in planta.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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