Nature Communications (Jun 2020)

In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors

  • Shey-Li Lim,
  • Chia Pao Voon,
  • Xiaoqian Guan,
  • Yi Yang,
  • Per Gardeström,
  • Boon Leong Lim

DOI
https://doi.org/10.1038/s41467-020-17056-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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NADP(H) and NAD(H) are crucial energy molecules in plant metabolism. Here, via the use of circularly permutated fluorescent protein sensors, the authors demonstrate dynamic changes in NADPH and the NADH/NAD+ ratio during photosynthesis and photorespiration at the subcellular level in planta.