Journal of Veterinary Medical Research (Jun 2007)
Determination of electrophoretic pattern of infectious bovine rhinotrachities virus of cattle
Abstract
A total number of 80 nasal swabs collected from apparently normal cattle slaughtered in Basateen abattoir were screened for the presence of infectious bovine rhinotracheitis virus. Among 80 examined samples, 4 samples found positive after the 3rd passage on MDBK cell line with appearance of the specific cytopathic effect (grape like clusters). The isolated virus titers were 103.9, 104.2, 105, 105.6 TCID50 / 0.1 ml. The four positive isolates were identified by agar gel precipitation test (AGPT), virus neutralization test (VNT) and gave the intracytoplasmic granules by indirect fluorescent antibody technique (IFAT). Electrophoretic profile of IBR in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was described and visualized by Coomassie blue stain. The mobilities of electrophoretic bands were determined with molecular weight marker at approximate range from 206.39 to 22.14 kDa.
