Frontiers in Genetics (Nov 2019)

Single Nucleotide Polymorphisms in Starch Biosynthetic Genes Associated With Increased Resistant Starch Concentration in Rice Mutant

  • Selvakumar Gurunathan,
  • Selvakumar Gurunathan,
  • Bharathi Raja Ramadoss,
  • Venkataramana Mudili,
  • Chandranayaka Siddaiah,
  • Naveen Kumar Kalagatur,
  • Jutti Rajendran Kannan Bapu,
  • Chakrabhavi Dhananjaya Mohan,
  • Abdulaziz A. Alqarawi,
  • Abeer Hashem,
  • Elsayed Fathi Abd_Allah

DOI
https://doi.org/10.3389/fgene.2019.00946
Journal volume & issue
Vol. 10

Abstract

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Resistant Starch (RS), plays a crucial role in human health and nutrition by controlling glucose metabolism. RS or dietary fibre content in rice is low because it goes through a variety of process before it is ready for cooking and consumption. Hence, this study was carried out to develop a rice mutant with increased RS. The rice mutant (γ278) with increased RS was developed by utilizing gamma (γ) rays as a mutagen. Mutant γ278 was characterized for mutations in the starch biosynthetic genes viz., GBSSI, SSI, SSIIa, SSIIIa, SBEIa, and SBEIIb to reveal the functional mutations/variations led to high RS content in rice. A total of 31 sequence variants/mutations in six genes were identified. We report the discovery of three deleterious mutation/variants each in GBSSI, SSIIa, and SSIIIa with the potential to increase RS content in rice. Further, wild × mutant crosses were made to develop an F2 population to study the effect of combination of deleterious mutations. The SNP (GBSSI:ssIIa:ssIIIa) combination responsible for high RS content in F2 population was identified and recorded highest amylose content (AC) (26.18%) and RS (8.68%) content. In conclusion, this marker combination will be highly useful to develop a rice variety with increased RS.

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