Plasma FIB milling for the determination of structures in situ

Casper Berger; Maud Dumoux; Thomas Glen; Neville B.-y. Yee; John M. Mitchels; Zuzana Patáková; Michele C. Darrow; James H. Naismith; Michael Grange

doi:10.1038/s41467-023-36372-9

Nature Communications (Feb 2023)

Plasma FIB milling for the determination of structures in situ

Casper Berger,
Maud Dumoux,
Thomas Glen,
Neville B.-y. Yee,
John M. Mitchels,
Zuzana Patáková,
Michele C. Darrow,
James H. Naismith,
Michael Grange

Affiliations

Casper Berger: Structural Biology, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
Maud Dumoux: Structural Biology, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
Thomas Glen: Structural Biology, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
Neville B.-y. Yee: Artificial Intelligence & Informatics, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
John M. Mitchels: Thermo Fisher Scientific Brno s.r.o
Zuzana Patáková: Thermo Fisher Scientific Brno s.r.o
Michele C. Darrow: Artificial Intelligence & Informatics, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
James H. Naismith: Structural Biology, The Rosalind Franklin Institute, Harwell Science & Innovation Campus
Michael Grange: Structural Biology, The Rosalind Franklin Institute, Harwell Science & Innovation Campus

DOI: https://doi.org/10.1038/s41467-023-36372-9
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 12

Abstract

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The authors harness plasma focused ion beams for pseudo-atomic structure determination, reporting increased throughput and automation in in situ structural biology to elucidate structure-function relationships inside cells and tissues.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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