Evaluation of the presence of Campylobacter Jejuni and Listeria Monocytogenes in water sources of Kermanshah city before chlorination based on comparison of culture and PCR

Abstract

Abstract: Introduction: As the world population grows, it is predicted that freshwater supplies will be rare in the 21st century. Despite abundant advances in water and wastewater treatment, waterborne diseases still threaten the health of the people of the world. Listeria monocytogenes bacterium is a pathogen that causes listeriosis. This pathogen can also cause meningitis, poisonous sepsis, and abortion in humans. One way of transmitting this microorganism is water and foodstuffs. Quick and accurate identification plays an important role in preventing infections. Also, due to the importance of Campylobacter jejuni in water and food industries and causing infection, toxication, and digestive problems in humans, the identification of this bacterium can be an effective step in preventing water contamination with Campylobacter jejuni. The aim of the present study was to identify Listeria monocytogenes and Campylobacter jejuni through culture and PCR and compare them in the water supply of Kermanshah city.Methods: 18 samples were collected from different water supplies of Kermanshah. DNA was extracted from standard Campylobacter jejuni and Listeria monocytogenes using a DNG-Plus kit. PCR reaction was optimized using specific primers. After determining the specificity and PCR detection limit, the collected water samples were examined and at the same time, the samples were cultured and examined.Findings: From 18 samples of water supply sources in Kermanshah by PCR, Campylobacter jejuni was isolated from all samples, and Listeria monocytogenes was isolated from 17 samples, and also 4 cases of Campylobacter jejuni and 2 cases of Listeria monocytogenes were isolated by culture method. The results showed that PCR has a better performance than culture for detecting Listeria monocytogenes and Campylobacter jejuni.

Keywords

• listeria monocytogenes
• campylobacter jejuni
• pcr
• drinking water