Improving dryland maize productivity and water efficiency with heterotrophic ammonia-oxidizing bacteria via nitrification and cytokinin activity

Abstract

A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield. A heterotrophic ammonia-oxidizing bacteria (HAOB) strain S2_8_1 was used. Six treatments were applied: (1) no irrigation + HAOB strain (DI), (2) no irrigation + blank culture medium (DM), (3) no irrigation control (DCK), (4) irrigation + HAOB (WI), (5) irrigation + blank culture medium (WM), and (6) irrigation control (WCK). Results revealed that HAOB treatment increased maize growth, yield, and water use efficiency over controls, regardless of whether the year was wet or dry. This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation, which subsequently led to increased levels of leaf cytokinins. Overall, these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.

Keywords

• Heterotrophic ammonia-oxidizing bacteria
• Rhizosphere soil nitrification
• Cytokinin
• Maize
• Dryland agriculture