Trichothecenes B and ergosterol content evaluation in mature grains of durum wheat genotypes contaminated by Fusarium culmorum

Abstract

The interest of this present work is to compare two selected durum wheat lines (G1 and G4) with their sensitive parents to fusarium head blight by determining the mycotoxin and ergosterol content in their mature grains. These lines are homozygous, fixed, obtained by the classical genealogical selection, and composed of seeds from diallel crosses between 4 parental varieties Saadi, Simeto, Ardente, and Waha. For this purpose, the grains of the studied genotypes (lines and parental varieties) were tested in the laboratory for their content of Trichothecenes B mycotoxins (TCTB) and ergosterol at full grain maturity using high-performance liquid chromatographic – diode array detection (HPLC-DAD). Fungal biomass was estimated by the content of ergosterol. However, the level of toxins was assessed by the levels of TCTB produced by the different studied isolates. The results obtained showed that the lines derived from crosses and, especially the G1 line, accumulated levels of TCTB and ergosterol significantly lower than their parents. Our findings open up a new avenue of investigation into fusarium head blight in Algeria, including the search for mycotoxins as potential causes of poorly understood human diseases and the factors that contribute to their accumulation in grains.

Keywords

• durum wheat
• fusarium head blight
• algeria
• trichotecenes b
• ergosterol