Central Asian Journal of Medical Sciences (Mar 2018)

Regulating Action of in Vitro Hepatitis C Virus Infection on Interferon-Induced Interferon Stimulating Genes in Murine Macrophages

  • Dolgorsuren Sandagdorj,
  • Batkhishig Munkhjargal,
  • Baasansuren Enkhjargal,
  • Baljinnyam Tuvdenjamts,
  • Enkhjin Zorigt,
  • Budjav Jadamba,
  • Altanshagai Chinbat,
  • Khongorzul Samdankhuu,
  • Ulziisaikhan Jambalganii,
  • Batsuren Boldbaatar,
  • Tsogtsaikhan Sandag,
  • Bilegtsaikhan Tsolmon,
  • Enkhsaikhan Lkhagvasuren

DOI
https://doi.org/10.24079/cajms.2018.03.004
Journal volume & issue
Vol. 4, no. 1
pp. 25 – 34

Abstract

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Objectives: The objective of this study was to determine the in vitro regulating effect of Hepatitis C virus (HCV) infection on interferon stimulating genes (ISGs) stimulated by IFN-ү and Imiquimod (TLR7 ligand) on murine macrophages. iNOS and STAT1 were measured by RT-PCR, and immunoblotting (IB). Nitric oxide production was measured by Griess Reagent Assay. Objectives: The objective of this study was to determine the in vitro regulating effect of Hepatitis C virus (HCV) infection on interferon stimulating genes (ISGs) stimulated by IFN-ү and Imiquimod (TLR7 ligand) on murine macrophages. iNOS and STAT1 were measured by RT-PCR, and immunoblotting (IB). Nitric oxide production was measured by Griess Reagent Assay. Results: HCV inhibits IFN induced iNOS mRNA and also protein expression. HCV significantly reduced IFN-ү induced ISGs (iNOS mRNA, iNOS protein, s727-STAT1, tyr701- STAT1). Conclusion: These results indicate that in vitro hepatitis C virus infection is involved in the regulation of IFN-ү induced ISGs in the levels of gene and protein expression of iNOS and STAT1 transcription factors.: HCV inhibits IFN induced iNOS mRNA and also protein expression. HCV significantly reduced IFN-ү induced ISGs (iNOS mRNA, iNOS protein, s727-STAT1, tyr701- STAT1). Conclusion: These results indicate that in vitro hepatitis C virus infection is involved in the regulation of IFN-ү induced ISGs in the levels of gene and protein expression of iNOS and STAT1 transcription factors.

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