Microchip Immunoassays for Monitoring Renal Function: Rapid, Low-Cost, and Highly Sensitive Quantification of Urinary Biomarkers of Diabetic Nephropathy
Toshihiro Kasama,
Miaomiao Sun,
Noritada Kaji,
Shin’ichi Akiyama,
Yukio Yuzawa,
Manabu Tokeshi,
Seiichi Matsuo,
Yoshinobu Baba
Affiliations
Toshihiro Kasama
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, Japan
Miaomiao Sun
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Aichi 464-8603, Japan
Noritada Kaji
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Aichi 464-8603, Japan
Shin’ichi Akiyama
Division of Nephrology, Department of Internal Medicine, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan
Yukio Yuzawa
Department of Nephrology, Fujita Health University, Aichi 470-1192, Japan
Manabu Tokeshi
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Aichi 464-8603, Japan
Seiichi Matsuo
Division of Nephrology, Department of Internal Medicine, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan
Yoshinobu Baba
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Aichi 464-8603, Japan
This study developed low-cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP-1), angiotensinogen (AGT), and liver-type fatty acid binding protein (L-FABP). The devices were used to successfully estimate the concentrations of the three biomarkers in urine samples within 2 min; the results were consistent with those obtained via conventional enzyme-linked immunosorbent assay (ELISA), which requires several hours. In addition, the estimated detection limits for the three biomarkers were comparable to those of commercially available ELISA kits. Thus, the proposed and fabricated devices facilitate high-precision and frequent monitoring of renal function.
