Microorganisms (May 2021)

Genomic and Phenotypic Analysis of Linezolid-Resistant <i>Staphylococcus epidermidis</i> in a Tertiary Hospital in Innsbruck, Austria

  • Silke Huber,
  • Miriam A. Knoll,
  • Michael Berktold,
  • Reinhard Würzner,
  • Anita Brindlmayer,
  • Viktoria Weber,
  • Andreas E. Posch,
  • Katharina Mrazek,
  • Sarah Lepuschitz,
  • Michael Ante,
  • Stephan Beisken,
  • Dorothea Orth-Höller,
  • Johannes Weinberger

DOI
https://doi.org/10.3390/microorganisms9051023
Journal volume & issue
Vol. 9, no. 5
p. 1023

Abstract

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Whole genome sequencing is a useful tool to monitor the spread of resistance mechanisms in bacteria. In this retrospective study, we investigated genetic resistance mechanisms, sequence types (ST) and respective phenotypes of linezolid-resistant Staphylococcus epidermidis (LRSE, n = 129) recovered from a cohort of patients receiving or not receiving linezolid within a tertiary hospital in Innsbruck, Austria. Hereby, the point mutation G2603U in the 23S rRNA (n = 91) was the major resistance mechanism followed by the presence of plasmid-derived cfr (n = 30). The majority of LRSE isolates were ST2 strains, followed by ST5. LRSE isolates expressed a high resistance level to linezolid with a minimal inhibitory concentration of ≥256 mg/L (n = 83) in most isolates, particularly in strains carrying the cfr gene (p cfr in LRSE are potential key steps to reduce linezolid resistance and its transmission to more pathogenic Gram-positive bacteria.

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