Emerging Microbes and Infections (Dec 2022)

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

  • Jieming Qu,
  • Jing Zhang,
  • Yu Chen,
  • Yi Huang,
  • Yusang Xie,
  • Min Zhou,
  • Yuping Li,
  • Dongwei Shi,
  • Jinfu Xu,
  • Qiuyue Wang,
  • Bei He,
  • Ning Shen,
  • Bin Cao,
  • Danyang She,
  • Yi Shi,
  • Xin Su,
  • Hua Zhou,
  • Hong Fan,
  • Feng Ye,
  • Qiao Zhang,
  • Xinlun Tian,
  • Guoxiang Lai

DOI
https://doi.org/10.1080/22221751.2022.2035194
Journal volume & issue
Vol. 11, no. 1
pp. 556 – 566

Abstract

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Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into an online database. Blood, urine and respiratory specimens were collected for routine culture, antigen detection, PCR and mNGS as designed appropriately. Aetiology confirmation was made by the local attending physician group and scientific committee according to microbiological results, clinical features, and response to the treatment. Two hundred seventy-five patients were included for final analysis. Combined detection methods made identification rate up to 74.2% (222/299), while 14.4% (43/299) when only using routine cultures and 40.8% (122/299) when not using mNGS. Influenza virus (23.2%, 46/198), S. pneumoniae (19.6%, 39/198), Enterobacteriaceae (14.6%, 29/198), Legionella pneumophila (12.6%, 25/198), Mycoplasma pneumoniae (11.1%, 22/198) were the top five common pathogens. The in-hospital mortality of patients with pathogen identified and unidentified was 21.7% (43/198) and 25.9% (20/77), respectively. In conclusion, early combined detection increased the pathogen identification rate and possibly benefitted survival. Influenza virus, S. pneumoniae, Enterobacteriaceae was the leading cause of SCAP in China, and there was a clear seasonal distribution pattern of influenza viruses. Physicians should be aware of the emergence of uncommon pathogens, including Chlamydia Psittaci and Leptospira.

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