MICROMYCETES – POTENTIAL BIODESTRUCTORS OF RUBBER TECHNICAL MATERIALS WASTES IN THE CLAMATE OF UKRAINE

Abstract

Aim of the presented paper was the preliminary screening of microscopic fungi promising for development of the biotechnology of destruction of the wastes of rubber technical materials (RTM). Methods. The cultures of micromycetes were isolated from RTM reposited on agarized mash (4.5° sensu Balling) on 4 °С. An ability of investigated micromycetes to grow on RTM was estimated on the model caoutchouc-based medium (CBM) imitated rubber substrate. The presence of carboxylesterase and oxidases was educed by the qualitative methods and the enzymatic index was calculated. The compatibility of the selected cultures of micromycetes has been estimated by the method of agarinic blocks. Results. It has been shown that 77 micromycetes from 85 studied strains were able to form clear colonies on CBM. Among them only five strains – Aspergillus fumigatus F-41484, Mucor racemosus F-41411, M. racemosus F-41412, Rhizopus cohnii F-2 and R. cohnii F-3 have high enzymatic indexes by the carboxylesterase activity and five strains – Alternaria alternata F-41431, Aspergillus flavus F-41432, A. sydowii F-41426, A. ustus F-41437 and A. versicolor F-41469 – have high enzymatic indexes by the oxidase activity. It has been ascertained an ability of using of the strains that have high enzymatic indexes in the eight mixed cultures. Conclusion. Ten fungal strains that have been characterized by high enzymatic indexes for carboxylesterase or complex of oxidative enzymes and were able to combined cultivation, were selected for development of technology of biodestruction of RTM wastes.

Keywords

• гумотехнічні матеріали
• мікроміцети
• біодеструкція
• ферменти
• ензиматичний індекс
• змішана культура