TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

Kyle B. Lupo; Sandra Torregrosa-Allen; Bennett D. Elzey; Sagar Utturkar; Nadia A. Lanman; Aaron A. Cohen-Gadol; Veronika Slivova; MacKenzie McIntosh; Karen E. Pollok; Sandro Matosevic

iScience (Dec 2023)

TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

Kyle B. Lupo,
Sandra Torregrosa-Allen,
Bennett D. Elzey,
Sagar Utturkar,
Nadia A. Lanman,
Aaron A. Cohen-Gadol,
Veronika Slivova,
MacKenzie McIntosh,
Karen E. Pollok,
Sandro Matosevic

Affiliations

Kyle B. Lupo: Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN, USA
Sandra Torregrosa-Allen: Center for Cancer Research, Purdue University, West Lafayette, IN, USA
Bennett D. Elzey: Center for Cancer Research, Purdue University, West Lafayette, IN, USA; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA; Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA
Sagar Utturkar: Center for Cancer Research, Purdue University, West Lafayette, IN, USA
Nadia A. Lanman: Center for Cancer Research, Purdue University, West Lafayette, IN, USA; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA
Aaron A. Cohen-Gadol: Department of Neurological Surgery, Indiana University, Indianapolis, IN, USA
Veronika Slivova: Enterprise Clinical Research Operations Biorepository, Indiana University Health, Indianapolis, IN 46202, USA
MacKenzie McIntosh: Histology Research Laboratory, Center for Comparative Translational Research, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA
Karen E. Pollok: In Vivo Therapeutics Core, Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, USA
Sandro Matosevic: Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN, USA; Center for Cancer Research, Purdue University, West Lafayette, IN, USA; Corresponding author

Journal volume & issue: Vol. 26, no. 12
p. 108353

Abstract

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Summary: TIGIT is a receptor on human natural killer (NK) cells. Here, we report that TIGIT does not spontaneously induce inhibition of NK cells in glioblastoma (GBM), but rather acts as a decoy-like receptor, by usurping binding partners and regulating expression of NK activating ligands and receptors. Our data show that in GBM patients, one of the underpinnings of unresponsiveness to TIGIT blockade is that by targeting TIGIT, NK cells do not lose an inhibitory signal, but gains the potential for new interactions with other, shared, TIGIT ligands. Therefore, TIGIT does not define NK cell dysfunction in GBM. Further, in GBM, TIGIT+ NK cells are hyperfunctional. In addition, we discovered that 4-1BB correlates with TIGIT expression, the agonism of which contributes to TIGIT immunotherapy. Overall, our data suggest that in GBM, TIGIT acts as a regulator of a complex network, and provide new clues about its use as an immunotherapeutic target.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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