A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases

Alvaro Molina-Cruz; Nadia Raytselis; Roxanne Withers; Ankit Dwivedi; Peter D. Crompton; Boubacar Traore; Giovanna Carpi; Joana C. Silva; Carolina Barillas-Mury

doi:10.1038/s42003-021-02667-0

Communications Biology (Sep 2021)

A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases

Alvaro Molina-Cruz,
Nadia Raytselis,
Roxanne Withers,
Ankit Dwivedi,
Peter D. Crompton,
Boubacar Traore,
Giovanna Carpi,
Joana C. Silva,
Carolina Barillas-Mury

Affiliations

Alvaro Molina-Cruz: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Nadia Raytselis: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Roxanne Withers: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Ankit Dwivedi: Institute for Genome Sciences, University of Maryland School of Medicine
Peter D. Crompton: Malaria Infection Biology and Immunity Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, NIH
Boubacar Traore: Mali International Center of Excellence in Research, University of Sciences, Techniques and Technologies of Bamako
Giovanna Carpi: Department of Biological Sciences, Purdue University
Joana C. Silva: Institute for Genome Sciences, University of Maryland School of Medicine
Carolina Barillas-Mury: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

DOI: https://doi.org/10.1038/s42003-021-02667-0
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 6

Abstract

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Molina-Cruz et al. analyzed 4,971 sequences of the Plasmodium falciparum Pfs47 gene and identified a combination of two SNP haplotypes highly correlated with geographic origin and transmission potential by mosquito vectors. A simple High-Resolution Melting RT-PCR assay was developed to genotype these SNPs and establish the likely continent of origin and transmission risk of P. falciparum isolates.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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