A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells

Akshay K. Ahuja; Karolina Jodkowska; Federico Teloni; Anna H. Bizard; Ralph Zellweger; Raquel Herrador; Sagrario Ortega; Ian D. Hickson; Matthias Altmeyer; Juan Mendez; Massimo Lopes

doi:10.1038/ncomms10660

Nature Communications (Feb 2016)

A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells

Akshay K. Ahuja,
Karolina Jodkowska,
Federico Teloni,
Anna H. Bizard,
Ralph Zellweger,
Raquel Herrador,
Sagrario Ortega,
Ian D. Hickson,
Matthias Altmeyer,
Juan Mendez,
Massimo Lopes

Affiliations

Akshay K. Ahuja: Institute of Molecular Cancer Research, University of Zurich
Karolina Jodkowska: DNA Replication Group, Molecular Oncology Programme, CNIO
Federico Teloni: Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich
Anna H. Bizard: Department of Cellular and Molecular Medicine, Center for Chromosome Stability and Center for Healthy Aging, University of Copenhagen, Panum Institute
Ralph Zellweger: Institute of Molecular Cancer Research, University of Zurich
Raquel Herrador: Institute of Molecular Cancer Research, University of Zurich
Sagrario Ortega: Transgenic Mice Core Unit, Biotechnology Programme, CNIO
Ian D. Hickson: Department of Cellular and Molecular Medicine, Center for Chromosome Stability and Center for Healthy Aging, University of Copenhagen, Panum Institute
Matthias Altmeyer: Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich
Juan Mendez: DNA Replication Group, Molecular Oncology Programme, CNIO
Massimo Lopes: Institute of Molecular Cancer Research, University of Zurich

DOI: https://doi.org/10.1038/ncomms10660
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

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In fast proliferating embryonic stem cells (ESC) the DNA damage response is activated by mechanisms that are as yet elusive. Here, Ahuja et al.link the DNA damage response to replication stress in mouse ESCs, caused by a short G1 phase, and propose fork remodelling as maintaining genome stability in embryos.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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