Nature Communications (Sep 2019)
Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
- Josh Tycko,
- Michael Wainberg,
- Georgi K. Marinov,
- Oana Ursu,
- Gaelen T. Hess,
- Braeden K. Ego,
- Aradhana,
- Amy Li,
- Alisa Truong,
- Alexandro E. Trevino,
- Kaitlyn Spees,
- David Yao,
- Irene M. Kaplow,
- Peyton G. Greenside,
- David W. Morgens,
- Douglas H. Phanstiel,
- Michael P. Snyder,
- Lacramioara Bintu,
- William J. Greenleaf,
- Anshul Kundaje,
- Michael C. Bassik
Affiliations
- Josh Tycko
- Department of Genetics, Stanford University
- Michael Wainberg
- Department of Computer Science, Stanford University
- Georgi K. Marinov
- Department of Genetics, Stanford University
- Oana Ursu
- Department of Genetics, Stanford University
- Gaelen T. Hess
- Department of Genetics, Stanford University
- Braeden K. Ego
- Department of Genetics, Stanford University
- Aradhana
- Department of Genetics, Stanford University
- Amy Li
- Department of Genetics, Stanford University
- Alisa Truong
- Department of Genetics, Stanford University
- Alexandro E. Trevino
- Center for Personal Dynamic Regulomes, Stanford University
- Kaitlyn Spees
- Department of Genetics, Stanford University
- David Yao
- Department of Genetics, Stanford University
- Irene M. Kaplow
- Department of Computer Science, Stanford University
- Peyton G. Greenside
- Department of Genetics, Stanford University
- David W. Morgens
- Department of Genetics, Stanford University
- Douglas H. Phanstiel
- Department of Genetics, Stanford University
- Michael P. Snyder
- Department of Genetics, Stanford University
- Lacramioara Bintu
- Department of Bioengineering, Stanford University
- William J. Greenleaf
- Department of Genetics, Stanford University
- Anshul Kundaje
- Department of Genetics, Stanford University
- Michael C. Bassik
- Department of Genetics, Stanford University
- DOI
- https://doi.org/10.1038/s41467-019-11955-7
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 14
Abstract
Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.
